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Image Search Results
Journal: Molecular Cell
Article Title: MINERVA: A Facile Strategy for SARS-CoV-2 Whole-Genome Deep Sequencing of Clinical Samples
doi: 10.1016/j.molcel.2020.11.030
Figure Lengend Snippet: Scheme and Development of MINERVA (A) RNA extracted from pharyngeal swabs and sputum and stool samples undergo rRNA and DNA removal before metagenomic sequencing library construction (MINERVA-m). Multiple libraries were then pooled for SARS-CoV-2 sequence enrichment. (B) Effect of the N10 primer during reverse transcription and Tn5 amount on detected gene number. (C) Effect of the N10 primer during reverse transcription and Tn5 amount on gene body coverage evenness.
Article Snippet: After nucleic acids extraction, rRNA was removed by rDNA probe hybridization and RNase H digestion, followed by DNA removal through DNase I digestion, using
Techniques: Sequencing, Reverse Transcription